Feulgen method

Feulgen method
a method of demonstrating chromatin and deoxyribonucleic acid (DNA).

Medical dictionary. 2011.

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  • Feulgen reaction — n the development of a brilliant purple color by DNA in a microscopic preparation stained with a modified Schiff s reagent * * * a method of demonstrating the presence of DNA in cell nuclei. The tissue section under investigation is first… …   Medical dictionary

  • Feulgen reaction — a method of demonstrating the presence of DNA in cell nuclei. The tissue section under investigation is first hydrolysed with dilute hydrochloric acid and then treated with Schiff s reagent. A purple coloration develops in the presence of DNA… …   The new mediacal dictionary

  • F method — chromosomes are treated with phosphate buffer, rinsed and stored for 60–72 hours in saline citrate solution, then fixed in methanol acetic acid, and stained by the Feulgen method …   Medical dictionary

  • Robert Feulgen — (1884 1955) was a German chemist who, in 1914, developed a method for staining DNA (now known as the Feulgen stain) and who also discovered that DNA is located in the chromosomes …   Wikipedia

  • stain — 1. To discolor. 2. To color; to dye. 3. A discoloration. 4. A dye used in histologic and bacteriologic technique. 5. A procedure in which a dye or combination of dyes and reagents is used to color the constituents of cells and tissues. For… …   Medical dictionary

  • reaction — 1. The response of a muscle or other living tissue or organism to a stimulus. 2. The color change effected in litmus and certain other organic pigments by contact with substances such as acids or alkalies; also the property that such substances… …   Medical dictionary

  • cytometry — The counting of cells, especially blood cells, using a cytometer or hemocytometer. Feulgen c. a form of c. using Feulgen stained nuclei to characterize the chromatin pattern and nuclear distribution of DNA of cells. flow …   Medical dictionary

  • Reagent — A substance used to produce a chemical reaction to detect, measure, produce, etc. other substances. * * * Any substance added to a solution of another substance to participate in a chemical reaction. [Mod. L. reagens] amino acid r. a r. used in… …   Medical dictionary

  • J. F. A. McManus — Joseph Forde Anthony McManus, (July 13, 1911 ndash; March 4, 1980) was a Canadian pathologist who is best known for his formulation of one of the most frequently used stains in histopathology; the McManus Periodic acid Schiff stain. Joe McManus… …   Wikipedia

  • Torbjörn Caspersson — Infobox Scientist name = Torbjörn Caspersson box width = image size =150px caption = Torbjörn Caspersson birth date = October 15, 1910 birth place = Motala death date = December 7, 1997 death place = residence = citizenship = nationality =… …   Wikipedia

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