ligase chain reaction

ligase chain reaction
a type of DNA amplification technique that uses DNA ligase to link adjacent pairs of synthetic oligonucleotides that have been hybridized to complementary regions on each strand of the double-stranded target DNA, with the product used as a template for subsequent cycles of amplification of the sequence in question. Because the ligation reaction requires perfect base pairing, sequence variation at the ligation site can be demonstrated.

Medical dictionary. 2011.

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