formate–tetrahydrofolate ligase

for·mate–tet·ra·hy·dro·fo·late li·gase (forґmāt tet″rə-hi″dro-foґlāt liґgās) [EC 6.3.4.3] an enzyme activity of the ligase class that catalyzes the ATP-dependent synthesis of 10-formyltetrahydrofolate from formate and tetrahydrofolate, a step in the system of folate-mediated one-carbon transfer reactions. The enzyme activity is part of a trifunctional enzyme that also includes methenyltetrahydrofolate cyclohydrolase (q.v.) and methylenetetrahydrofolate dehydrogenase (NADP⁺) activities.