Blot, Southern

Blot, Southern
A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules. In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridized with complementary radiolabeled probes. The aim is to detect specific base sequences with the probes. Lest all of this sound esoteric, note that in the television series "The X Files" a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person’s genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot.

Medical dictionary. 2011.

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  • Southern blot — A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules. In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis,… …   Medical dictionary

  • blot — See Northern b. analysis, Southern b. analysis, Western b. analysis, zoo b. analysis. * * * Bimodality Lung Oncology Trial; British Library of Tape * * * blot blät n a nitrocellulose sheet that contains spots of immobilized macromolecules (as of… …   Medical dictionary

  • Southern blot — noun Etymology: Edwin M. Southern b1938 British biologist Date: 1979 a blot consisting of a nitrocellulose or nylon sheet containing spots of DNA for identification by a suitable molecular probe compare Western blot • Southern blotting noun …   New Collegiate Dictionary

  • Southern Blot — Un transfert d ADN[1] est une méthode de biologie moléculaire permettant l analyse de l ADN. Sommaire 1 Principe 2 Méthode 3 Résultat …   Wikipédia en Français

  • Southern blot — Southern blot, hibridación Southern o, simplemente, Southern es un método de biología molecular que permite detectar la presencia de una secuencia de ADN en una mezcla compleja de este ácido nucleico. Para ello, emplea la técnica de… …   Wikipedia Español

  • Southern Blotting — Southern blot Un transfert d ADN[1] est une méthode de biologie moléculaire permettant l analyse de l ADN. Sommaire 1 Principe 2 Méthode 3 Résultat …   Wikipédia en Français

  • Southern — (englisch für „südlich“) steht für: Southern (Bahnbetriebsgesellschaft), derzeitige Eisenbahngesellschaft im Vereinigten Königreich Southern Blot, eine molekularbiologische Methode zum Nachweis spezifischer DNA Sequenzen in einem DNA Gemisch… …   Deutsch Wikipedia

  • Southern-Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA–Gemisch… …   Deutsch Wikipedia

  • Southern Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für die DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA… …   Deutsch Wikipedia

  • Southern blot — A Southern blot is a method routinely used in molecular biology to check for the presence of a DNA sequence in a DNA sample. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size… …   Wikipedia

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