Southern blot

Southern blot
A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules. In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridized with complementary radiolabeled probes. The aim is to detect specific base sequences with the probes. Lest all of this sound esoteric, note that in the television series "The X Files" a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person’s genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot.

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South·ern blot 'səth-ərn- n a blot consisting of a sheet of cellulose nitrate or nylon that contains spots of DNA for identification by a suitable molecular probe compare NORTHERN BLOT, WESTERN BLOT
Southern blot·ting -'blät-iŋ n
Southern Edwin M. (fl 1975)
British molecular biologist. In 1975 Southern delineated a method for identifying specific DNA sequences that had been previously separated by gel electrophoresis.

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[E.M. Southern] a blot obtained by transferring denatured DNA fragments separated by agarose gel electrophoresis to a nitrocellulose or nylon membrane, followed by hybridization to a labeled DNA or RNA probe; used to detect and analyze specific DNA sequences.

Medical dictionary. 2011.

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  • Southern Blot — Un transfert d ADN[1] est une méthode de biologie moléculaire permettant l analyse de l ADN. Sommaire 1 Principe 2 Méthode 3 Résultat …   Wikipédia en Français

  • Southern blot — Southern blot, hibridación Southern o, simplemente, Southern es un método de biología molecular que permite detectar la presencia de una secuencia de ADN en una mezcla compleja de este ácido nucleico. Para ello, emplea la técnica de… …   Wikipedia Español

  • Southern-Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA–Gemisch… …   Deutsch Wikipedia

  • Southern Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für die DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA… …   Deutsch Wikipedia

  • Southern blot — A Southern blot is a method routinely used in molecular biology to check for the presence of a DNA sequence in a DNA sample. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size… …   Wikipedia

  • Southern blot — Un transfert d ADN[1] est une méthode de biologie moléculaire permettant l analyse de l ADN. Elle a été inventée par Edwin Southern, un professeur britannique de biologie moléculaire. C est ce nom qui a, par jeu de mot, inspiré l appellation de… …   Wikipédia en Français

  • Southern blot — 1. noun A sheet to which DNA has been transfered from electrophoresis gel. 2. verb To use a Southern blot on (some DNA) …   Wiktionary

  • Southern blot analysis — see Southern blot, under blot …   Medical dictionary

  • Southern blot hybridization — see Southern blot, under blot …   Medical dictionary

  • southern blot — noun Usage: usually capitalized S Etymology: after Edwin M. Southern b1938 British biologist : a blot consisting of a sheet of cellulose nitrate or nylon that contains spots of DNA for identification by a suitable molecular probe compare northern …   Useful english dictionary

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