In situ hybridization

In situ hybridization
The use of a DNA or RNA probe to detect the complementary sequence. In situ hybridization is like all nucleic acid hybridization in being a technique in which single-stranded nucleic acids (DNA or RNA) are permitted to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. Through nucleic acid hybridization, the degree of sequence identity can be determined and specific sequences detected and sometimes those sequences can be located on a specific chromosome. In situ hybridization is done for this purpose. It involves hybridizing a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localization of genes to specific chromosomes (via fluorescent in situ hybridization, or FISH analysis).

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nucleic acid hybridization in which a labeled, single-stranded nucleic acid probe is applied to prepared cells or histologic sections and annealing occurs in situ; performed to analyze the intracellular or intrachromosomal distribution, transcription, or other characteristics of the nucleic acid.

Medical dictionary. 2011.

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  • In situ hybridization — (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue ( in situ ), or, if the tissue is small enough (e.g. plant seeds,… …   Wikipedia

  • in situ hybridization — hibridizacija in situ statusas T sritis augalininkystė apibrėžtis Specifinių DNR sekų atpažinimo metodas sveikose chromosomose su radioaktyviu papildomuoju (komplementariuoju) zondu. atitikmenys: angl. in situ hybridization rus. гибридизация in… …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • in situ hybridization —  In situ hybridization  Гибридизация in situ   Метод, использующий одноцепочечный РНК или ДНК зонд для обнаружения гена или молекулы информационной РНК в клетке или ткани (см. также гибридизация) …   Толковый англо-русский словарь по нанотехнологии. - М.

  • in-situ hybridization — noun (biology) A technique, using a radioactive nucleic acid probe and autoradiography, for locating the position of a specific DNA sequence on a chromosome • • • Main Entry: ↑in situ …   Useful english dictionary

  • in situ hybridization — in situ hybridization. См. цитологическая гибридизация. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • in situ hybridization — (1) Technique for revealing patterns of gene expression in a tissue. The tissue is fixed and prepared (usually by sectioning) on a slide, and a labelled DNA or RNA probe is hybridized to the sample. It binds only to complementary mRNA sequences,… …   Dictionary of molecular biology

  • Fluorescence in situ hybridization — A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the… …   Wikipedia

  • Fluorescent in situ hybridization — can be used to find out where the fluorescent probe bound to the chromosome. FISH is often used for finding specific features in DNA. These features can be used in genetic counseling, medicine, and species identification. Probes Probes are often… …   Wikipedia

  • Fluorescent in situ hybridization — Hybridation fluorescente in situ Pour les articles homonymes, voir FISH. Technique de l hybridation fluorescente in situ. En A : sonde. B : sonde colorée à l aide d un …   Wikipédia en Français

  • Fluorescent in situ hybridization — An important molecular cytogenetic method for identifying chromosomes and parts of chromosomes, deciphering chromosome rearrangements, and locating genes on chromosomes. Fluorescent means emitting light that comes from a reaction within the… …   Medical dictionary

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