- An immunohistochemical technique using labeling of antibodies by a fluorescent dye to identify antigenic material specific for the labeled antibody; the specific binding of antibody can be determined microscopically through the production of a characteristic visible light by the application of ultraviolet rays to the preparation. SEE ALSO: fluorescent antibody technique.- direct i. fluorescence microscopy of tissue from lesions after application of labeled antibodies. SEE ALSO: fluorescent antibody technique.- indirect i. fluorescence microscopy of normal tissue after application of the patient's serum, to detect antibodies to normal tissue components (autoantibodies). SEE ALSO: fluorescent antibody technique.
* * *im·mu·no·flu·o·res·cence -(.)flu̇(-ə)r-'es-ən(t)s, -flōr-, -flȯr- n the labeling of antibodies or antigens with fluorescent dyes esp. for the purpose of demonstrating the presence of a particular antigen or antibody in a tissue preparation or smearim·mu·no·flu·o·res·cent -ənt adj
* * *n.a technique for observing the amount and/or distribution of antibody or antigen in a tissue section. The antibodies are labelled (directly or indirectly) with a fluorescent dye (e.g. fluorescein) and applied to the tissue, which is observed through an ultraviolet microscope. In direct immunofluorescence the antibody is labelled before being applied to the tissue. In indirect immunofluorescence the antibody is labelled after it has bound to the antigen, by means of fluorescein-labelled anti-immunoglobulin serum.• immunofluorescent adj.
* * *im·mu·no·flu·o·res·cence (im″u-no-fl-resґəns) any immunohistochemical method that uses antibody labeled with a fluorescent dye.
Immunofluorescence (green) used to detect pemphigus vulgaris.
Medical dictionary. 2011.