hematoxylin

hematoxylin
A crystalline compound, containing the coloring matter of Haematoxylon campechianum (logwood), from which it is obtained by extraction with ether. It is used as a dye in histology, especially for cell nuclei and chromosomes, muscle cross-striations, and enterochromaffin cells; its staining properties depend upon its oxidation to hematein and mordanting with chrome and iron alums. It is also used as an indicator (red to yellow at pH 0.0 to 1.0, yellow to violet at pH 5.0 to 6.0).
- Boehmer h. an alum type of h. in which natural ripening occurs in about 8 to 10 days, and the solution is good for many months.
- Delafield h. an alum type of h. used in histology; natural ripening takes about 2 months and the solution is good for years.
- Harris h. an alum type of h. similar to Delafield h., but which uses chemical ripening to produce oxidation of h. for immediate use.
- iron h. unique ferric lakes of hematein that produce deep blue-black stains; useful for studies of cytologic detail, such as chromosomes, spindle fibers, Golgi apparatus, myofibrils, and mitochrondria; also useful to demonstrate Entamoeba histolytica. SEE ALSO: Heidenhain iron h. stain, Weigert iron h. stain.
- phosphotungstic acid h. (PTAH) a stain with broad application in cytology and histology; nuclei, mitochrondria, fibrin, neuroglial fibrils, and cross-striations of skeletal and cardiac muscle stain blue; cartilage ground substance, bone reticulum, and elastin appear in shades of yellow-orange and brownish red; also useful for demonstrating abnormal or diseased astrocytes, often in combination with periodic acid -Schiff stain and Luxol fast blue. SYN: Mallory phosphotungstic acid h. stain.

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he·ma·tox·y·lin or chiefly Brit hae·ma·tox·y·lin .hē-mə-'täk-sə-lən n a crystalline phenolic compound C16H14O6 found in logwood and used chiefly as a biological stain because of its ready oxidation to hematein

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he·ma·tox·y·lin (he″mə-tokґsĭ-lin) a colorless crystalline compound obtained by extracting logwood (Haematoxylon campechianum) with ether. It may be used as an indicator with a pH range of 5–6, but is mainly used in oxidized form as a stain in microscopy. See also at Stains and Staining Methods, under stain.

Medical dictionary. 2011.

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Look at other dictionaries:

  • Hematoxylin — Hem a*tox y*lin, n. H[ae]matoxylin. [1913 Webster] …   The Collaborative International Dictionary of English

  • hematoxylin — [hē΄mə täk′sə lin΄, hem΄ətäk′sə lin΄] n. [ H(A)EMATOXYL(ON) + IN1] a colorless, crystalline compound, C16H14O6·3H2O, extracted from logwood and used as an indicator and a stain in microscopy: when oxidized it yields hematein dye …   English World dictionary

  • hematoxylin — hematoksilinas statusas T sritis chemija apibrėžtis Indikatorius. formulė C₁₆H₁₄O₆ atitikmenys: angl. hematoxylin rus. гематоксилин …   Chemijos terminų aiškinamasis žodynas

  • hematoxylin-eosin stain — a mixture of hematoxylin in distilled water and aqueous eosin, usually eosin Y, solution, widely employed for routine examination of tissues; numerous variations are employed in execution of the stain. Hematoxylin eosin stain showing a glandular… …   Medical dictionary

  • hematoxylin — noun Etymology: International Scientific Vocabulary, from New Latin Haematoxylon, plant genus Date: circa 1847 a crystalline phenolic compound C16H14O6 found in logwood and used chiefly in biological staining …   New Collegiate Dictionary

  • hematoxylin — hematoxylic /hee meuh tok sil ik, hem euh /, adj. /hee meuh tok seuh lin, hem euh /, n. a colorless or pale yellow, crystalline compound, C16H14O63H2O, the coloring material of logwood: used as a mordant dye and as an indicator. [1840 50; HEMATO… …   Universalium

  • hematoxylin — noun A phenolic compound having the chemical formula CHO, used to make dye for staining tissue samples. Extracted from the heart of the logwood tree, Haematoxylum campechianum …   Wiktionary

  • hematoxylin — n. crystalline substance obtained from logwood and used in the production of various dyes and stains (Chemistry) …   English contemporary dictionary

  • hematoxylin — he·ma·tox·y·lin …   English syllables

  • hematoxylin — he•ma•tox•y•lin [[t]ˌhi məˈtɒk sə lɪn[/t]] n. chem. a colorless or pale yellow, crystalline compound, C16H14O6•3H2O, the coloring material of logwood: used as a mordant dye and as an indicator • Etymology: 1840–50; hemato + Gkxýl(on)wood + in I… …   From formal English to slang

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