stable transfection
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stable transfection — (= stable expression) When transfecting animal cells, a clone of cells in which the transgene has been physically incorporated into the genome. It thus provides stable, long term expression at the cost of being more difficult to produce … Dictionary of molecular biology
Transfection — is the process of introducing nucleic acids into cells by non viral methods [http://www.promega.com/guides/transfxn guide/transfxn.pdf] . The term transformation is preferred to describe non viral DNA transfer in bacteria and non animal… … Wikipedia
transfection — The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cell s chromosomal DNA. Usually accomplished using DNA precipitated with calcium ions though a variety of other methods can be used (eg.… … Dictionary of molecular biology
transfection — A method of gene transfer utilizing infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell. [trans + infection] * * * trans·fec·tion … Medical dictionary
Transfection — On appelle transfection le processus de transfert de gènes, c est à dire l introduction d ADN exogène dans des cellules eucaryotes, non médié par un virus, par opposition à la transduction. À noter que le terme « transfection » est… … Wikipédia en Français
Optical transfection — is the process of introducing nucleic acids into cells using light. Typically, a laser is focussed to a diffraction limited spot ( 1 µm diameter) using a high numerical aperture microscope objective. The plasma membrane of a cell is then… … Wikipedia
Molecular biology — (pronounced /məˈlɛkjʊlər .../) is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology… … Wikipedia
RNA interference — (RNAi) is a mechanism that inhibits gene expression at the stage of translation or by hindering the transcription of specific genes. RNAi targets include RNA from viruses and transposons (significant for some forms of innate immune response), and … Wikipedia
Chinese hamster ovary cell — Chinese hamster ovary (CHO) cells are a cell line derived from the ovary of the Chinese hamster. They are often used in biological and medical research and commercially in the production of therapeutic proteins.[1] They were introduced in the… … Wikipedia
List of molecular biology topics — This is a list of topics in molecular biology. See also list of biochemistry topics. NOTOC # 3 end 3 flanking region 5 end 5 flanking region 5 ribose 3 A acrylamide gels adenine adenosine deaminase deficiency adenovirus agarose gel… … Wikipedia
