Apt test

Apt test
(for differentiating fetal from adult hemoglobin) a specimen from an infant's vomitus or stool is mixed with 5 volumes of water and centrifuged so that a clear pink supernatant separates. Sodium hydroxide solution is added to the supernatant; if hemoglobin F (fetal blood) is present, the pink color persists for more than 2 minutes, whereas if hemoglobin A (from swallowed maternal blood) is present, the supernatant turns from pink to yellow within 2 minutes.

Medical dictionary. 2011.

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