acidified serum test

acidified serum test
(for paroxysmal nocturnal hemoglobinuria) the patient's washed red cells are incubated at 37°C in acidified normal serum or the patient's acidified serum; after centrifugation the supernatant is examined colorimetrically for hemolysis. In paroxysmal nocturnal hemoglobinuria the red cells are abnormally susceptible to lysis by complement, which is activated by the alternate pathway in acidified serum. Called also Ham t.

Medical dictionary. 2011.

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