Ehrlich diazo reagent

Ehrlich diazo reagent
Solution A: dissolve 5 g of sodium nitrite in 1 L of distilled water. Solution B: dissolve 5 g of sulfanilic acid and 50 mL of hydrochloric acid in 1 L of distilled water. For use mix 1 part of A with 50 to 100 parts of B.

Medical dictionary. 2011.

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  • Reagent — A substance used to produce a chemical reaction to detect, measure, produce, etc. other substances. * * * Any substance added to a solution of another substance to participate in a chemical reaction. [Mod. L. reagens] amino acid r. a r. used in… …   Medical dictionary

  • Ehrlich — Paul, German bacteriologist, immunologist, and Nobel laureate, 1854–1915. See Ehrlichia, E. anemia, E. inner body, E. phenomenon, E. postulate, E. diazo reagent, E. theory, E. Türk line. See entries under stain; reaction …   Medical dictionary

  • bilirubin — A yellow bile pigment found as sodium bilirubinate (soluble), or as an insoluble calcium salt in gallstones; formed from hemoglobin during normal and abnormal destruction of erythrocytes by the reticuloendothelial …   Medical dictionary

  • azobilirubin — The red violet pigment formed by the condensation of diazotized sulfanilic acid with bilirubin in the van den Bergh reaction. * * * azo·bil·i·ru·bin (a″zo bilґĭ roo″bin) bilirubin that has been diazotized by exposure to Ehrlich… …   Medical dictionary

  • reaction — 1. The response of a muscle or other living tissue or organism to a stimulus. 2. The color change effected in litmus and certain other organic pigments by contact with substances such as acids or alkalies; also the property that such substances… …   Medical dictionary

  • diazobenzenesulfonic acid — di·azo·ben·zene·sul·fon·ic acid or chiefly Brit di·azo·ben·zene·sul·phon·ic acid .dī .az (.)ō .ben .zēn .səl .fän ik n a white or reddish crystalline acid derivative C6H4N2O3S of sulfanilic acid that is used as the reagent in the diazo reaction * …   Medical dictionary

  • sulfanilic acid — sul·fa·nil·ic acid or chiefly Brit sul·pha·nil·ic acid .səl fə .nil ik n a crystalline acid C6H7NO3S obtained from aniline and used esp. in making dyes and as a reagent * * * sul·fa·nil·ic ac·id (sul″fə nilґik) a white crystalline… …   Medical dictionary

  • Jendrassik-Grof method — 1. (for conjugated bilirubin) a fasting sample of serum or plasma is collected and acidified by the addition of hydrochloric acid. Ehrlich s diazo reagent is added so that the conjugated bilirubin begins forming blue azobilirubin. After 10… …   Medical dictionary

  • stain — 1. To discolor. 2. To color; to dye. 3. A discoloration. 4. A dye used in histologic and bacteriologic technique. 5. A procedure in which a dye or combination of dyes and reagents is used to color the constituents of cells and tissues. For… …   Medical dictionary

  • test — 1. To prove; to try a substance; to determine the chemical nature of a substance by means of reagents. 2. A method of examination, as to determine the presence or absence of a definite disease or of some substance in any of the fluids, tissues,… …   Medical dictionary

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