subtractive hybridization

subtractive hybridization
a technique for isolating sequences expressed in one cell type but not in another; when a cDNA population prepared from the mRNA of one cell type is hybridized to the mRNA population of the second, and the cDNA:mRNA hybrids removed, the remaining single-stranded cDNA molecules represent the mRNA sequences expressed only in the first cell type.

Medical dictionary. 2011.

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