avidin-biotin complex

avidin-biotin complex
(ABC), avidin-biotin peroxidase complex a complex of avidin and biotinylated horseradish peroxidase that is added to a sample that has been treated with primary antibody specific for the antigen of interest and then with a biotinylated secondary antibody, which binds to an antigenic determinant on the primary antibody, so that several horseradish peroxidase molecules are localized to the site of the primary antigen. It is used in a number of immunocytochemical and immunohistochemical techniques.

Medical dictionary. 2011.

Игры ⚽ Нужно решить контрольную?

Look at other dictionaries:

  • Avidin — Biological Overview= Avidin is a tetrameric protein produced in the oviducts of birds, reptiles and amphibians which is deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein… …   Wikipedia

  • Biotin — A water soluble B complex vitamin involved in carbon dioxide transfer and therefore essential to the metabolism of carbohydrate and fat. A balanced diet usually contains enough biotin. Foods with high biotin levels include nuts, cereals, green… …   Medical dictionary

  • Biotin — Biotin[1] …   Wikipedia

  • biotin — /buy euh tin/, n. Biochem. a crystalline, water soluble vitamin, C10H16O3N2S, of the vitamin B complex, that is present in all living cells and functions as a growth factor and as a catalyst in carboxylation reactions. Also called vitamin H.… …   Universalium

  • biotin — n. a vitamin of the B complex that is essential for the metabolism of fat, being involved in fatty acid synthesis and gluconeogenesis. A biotin deficiency is extremely rare in humans; it can be induced by eating large quantities of raw egg white …   The new mediacal dictionary

  • biotin — A vitamin of the B complex, it acts as a co enzyme for various enzymes that catalyse the incorporation of carbon dioxide into various compounds, and is essential for the metabolism of fats. Adequate amounts are normally produced by the intestinal …   Glossary of Biotechnology

  • Meir Wilchek — Born 1935 Warsaw, Poland …   Wikipedia

  • NeutrAvidin — protein is a deglycosylated version of avidin, with a mass of approximately 60,000 daltons. As a result of carbohydrate removal, lectin binding is reduced to undetectable levels, yet biotin binding affinity is retained because the carbohydrate is …   Wikipedia

  • Dissociation constant — Kd redirects here. For other uses, see KD (disambiguation). In chemistry, biochemistry, and pharmacology, a dissociation constant is a specific type of equilibrium constant that measures the propensity of a larger object to separate (dissociate)… …   Wikipedia

  • ABC — abacavir; absolute basophil count; absolute bone conduction; acalculous biliary colic; acid balance control; aconite belladonna chloroform; adenosine triphosphate [ATP] binding cassette; airway, breathing, and circulation; alignment, blue,… …   Medical dictionary

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”