avidin-biotin complex

avidin-biotin complex
(ABC), avidin-biotin peroxidase complex a complex of avidin and biotinylated horseradish peroxidase that is added to a sample that has been treated with primary antibody specific for the antigen of interest and then with a biotinylated secondary antibody, which binds to an antigenic determinant on the primary antibody, so that several horseradish peroxidase molecules are localized to the site of the primary antigen. It is used in a number of immunocytochemical and immunohistochemical techniques.

Medical dictionary. 2011.

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