subtraction cloning

subtraction cloning
a type of DNA cloning in which the sequences to be cloned are first isolated by removal of some or all undesirable sequences from a heterogenous DNA population. It is performed by hybridization of a nucleic acid population containing the differentially expressed sequences of interest to an excess of a complementary nucleic acid population that lacks these sequences, then removing hybridized molecules and so enriching for the specific sequences, repeating until all sequences common to both populations are removed; the nucleic acid of interest can then be used to prepare or screen a library.

Medical dictionary. 2011.

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