hemolytic plaque assay

hemolytic plaque assay
a quantitative assay that counts antibody-producing cells. Lymphocytes sensitized against sheep erythrocytes (SRBCs) are plated in agar with SRBCs. After incubation complement is added; this lyses SRBCs, leaving a clear circular plaque around each cell that produced antibody against SRBC. The plaques are counted and reported as the number of plaque-forming cells (PFCs, pfcs). Called also Jerne plaque a.

Medical dictionary. 2011.

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