gel retardation assay

gel retardation assay
an assay used to determine whether a specific protein binds to DNA or to determine some characteristics of such binding; protein is incubated with or without nucleic acid under various conditions and the electrophoretic mobilities of the samples are compared. In a nondenaturing type of gel electrophoresis, a decreased rate of migration relative to an unbound control is indicative of binding.

Medical dictionary. 2011.

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