negative staining

negative staining
negative staining n a method of demonstrating the form of small objects (as bacteria) by surrounding them with a stain that they do not take up so that they appear as sharply outlined unstained bright bodies on a colored ground

* * *

staining of the background and not the organism, to facilitate the microscopical study of bacteria.

Medical dictionary. 2011.

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  • negative staining — negative staining. См. негативное окрашивание. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • negative staining — Microscopic technique in which the object stands out against a dark background of stain. For electron microscopy the sample is suspended in a solution of an electron dense stain such as sodium phosphotungstate and then sprayed onto a support grid …   Dictionary of molecular biology

  • negative staining — noun : a method of demonstrating the form of small objects (as bacteria) by surrounding them with a stain that they do not take up so that they appear as sharply outlined unstained bright bodies on a colored ground * * * negative staining, the… …   Useful english dictionary

  • negative staining — A staining procedure in which a dye is used to make the background dark while the specimen is unstained …   Dictionary of microbiology

  • Staining — is an auxiliary technique used in microscopy to enhance contrast in the microscopic image.In biochemistry it involves adding a class specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a… …   Wikipedia

  • Negative stain — Electron micrograph of (organic) plant tissue without (top) and with OsO4 staining Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. For bright field… …   Wikipedia

  • Gram staining — (or Gram s method) is an empirical method of differentiating bacterial species into two large groups (Gram positive and Gram negative) based on the chemical and physical properties of their cell walls.cite book | last = Bergey | first = David H.… …   Wikipedia

  • Gram-negative bacteria — Microscopic image of Gram negative Pseudomonas aeruginosa bacteria (pink red rods). Gram negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol.[1] In a Gram …   Wikipedia

  • Gram-negative — bacteria lose the crystal violet stain (and take the color of the red counterstain) in Gram s method of staining. This is characteristic of bacteria that have a cell wall composed of a thin layer of a particular substance (called peptidoglycan).… …   Medical dictionary

  • Differential staining — is a general term that can refer to a number of specific processes. Generally, it is used to describe staining processes which use more than one chemical stain. Using multiple stains can better differentiate between different microorganisms or… …   Wikipedia

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