Shotgun sequencing

Shotgun sequencing
An approach used to decode a genome by shredding ("shotgunning") it into smaller fragments of DNA which can then be individually sequenced. The sequences of these fragments are then ordered, based on overlaps in the genetic code, and finally reassembled into the complete sequence. The "whole genome shotgun" method is applied to the entire genome all at once, while the "hierarchical shotgun" method is applied to large, overlapping DNA fragments of known location in the genome.

Medical dictionary. 2011.

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  • Shotgun Sequencing — bzw. Schrotschusssequenzierung ist in der Molekularbiologie eine Methode zur Sequenzierung langer DNA Stränge. Sie wurde von Frederick Sanger 1982 entwickelt. Hierbei wird die DNA mehrfach kopiert und die Kopien werden zufällig in zahlreiche… …   Deutsch Wikipedia

  • Shotgun sequencing — In genetics, shotgun sequencing, also known as shotgun cloning, is a method used for sequencing long DNA strands. It is named by analogy with the rapidly expanding, quasi random firing pattern of a shotgun.Since the chain termination method of… …   Wikipedia

  • shotgun sequencing — noun A DNA sequencing technique in which a large number of small fragments of a long DNA strand are generated at random, sequenced, and reassembled to form a sequence of the original strand …   Wiktionary

  • Shotgun proteomics — is a method of identifying proteins in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry.cite journal |author=Hu L, Ye M, Jiang X, Feng S, Zou H |title=Advances in hyphenated analytical …   Wikipedia

  • whole-genome shotgun sequencing — An approach to genome sequencing in which the complete genome is broken into random fragments, which are then individually sequenced. Finally the fragments are placed in the proper order using sophisticated computer programs …   Dictionary of microbiology

  • Shotgun (disambiguation) — A shotgun may refer to:*Shotgun, a firearm *Shotgun (cannabis), one of a number of terms in the slang of cannabis users * Shotgun (film), a 1955 B Western starring Sterling Hayden * Shotgun , a song by the Canadian alternative rock band Moist *… …   Wikipedia

  • Shotgun lipidomics — In lipidomics, the process of shotgun lipidomics (named by analogy with shotgun sequencing uses analytical chemistry to investigate the biological function, significance, and sequelae of alterations in lipids and protein constituents mediating… …   Wikipedia

  • Shotgun — Der Begriff Shotgun (von engl. shotgun = Schrotflinte) bezeichnet: eine Flinte eine molekularbiologische Methode zur Entschlüsselung von Genomen, siehe Shotgun Sequencing einen Haustyp, der vor allem im Süden der USA verbreitet ist, siehe Shotgun …   Deutsch Wikipedia

  • shotgun genome sequencing — A strategy for sequencing a whole genome, in which the genomic DNA is initially fragmented into pieces small enough to be sequenced. Specialized computer software is then used to piece together the individual sequences to create long contiguous… …   Glossary of Biotechnology

  • Full genome sequencing — Genome sequencing redirects here. For the sequencing only of DNA, see DNA sequencing. An image of the 46 chromosomes, making up the diploid genome of human male. (The mitochondrial chromosome is not shown.) Full genome sequencing (FGS), also… …   Wikipedia

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