Endonuclease

Endonuclease
An enzyme that cleaves a nucleic acid (DNA or RNA) at specific internal sites in the nucleotide base sequence.
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An enzyme (phosphodiesterase) that cleaves the internal phosphodiester bonds in a DNA molecule, thus producing DNA fragments of varying size. Cf.:exonuclease.
- micrococcal e. an enzyme, produced by a member of the genus Micrococcus, that cleaves nucleic acid s to oligonucleotides terminating in 3′-phosphates. SYN: micrococcal nuclease, spleen e., spleen phosphodiesterases.
- nucleate e. SYN: e. Serratia marcescens.
- restriction e. one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a “chemical knife”; usually named by a three- or four-letter abbreviation of the name of the organism from which isolated ( e.g., EcoB from Escherichia coli, strain B). SYN: restriction enzyme.
- single-stranded nucleate e. e. S1 Aspergillus.
- spleen e. SYN: micrococcal e..

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en·do·nu·cle·ase -'n(y)ü-klē-.ās, -.āz n an enzyme that breaks down a nucleotide chain into two or more shorter chains by cleaving the internal phosphodiester bonds see RESTRICTION ENDONUCLEASE compare EXONUCLEASE

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en·do·nu·cle·ase (en″do-nooґkle-ās) any nuclease specifically catalyzing the hydrolysis of interior bonds of ribonucleotide or deoxyribonucleotide chains, producing poly- or oligonucleotides. Cf. exonuclease.

Medical dictionary. 2011.

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  • Endonuclease — Endonucléase Une endonucléase est une nucléase, qui coupe un acide nucléique en fragments plus courts. Les endonucléases sont capables de couper au milieu de la chaîne, par opposition aux exonucléases qui n attaquent que les nucléotides situés… …   Wikipédia en Français

  • endonuclease — endonuclease. См. эндонуклеаза. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Endonuclease — Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain, in contrast to exonucleases, which cleave phosphodiester bonds at the end of a polynucleotide chain. Restriction endonucleases (Restriction Enzymes)… …   Wikipedia

  • Endonucléase — Une endonucléase est une nucléase, qui coupe un acide nucléique en fragments plus courts. Les endonucléases sont capables de couper au milieu de la chaîne, par opposition aux exonucléases qui n attaquent que les nucléotides situés aux extrémités… …   Wikipédia en Français

  • endonuclease — endonukleazė statusas T sritis chemija apibrėžtis Nukleazė, katalizuojanti fosfodiesterinių ryšių, esančių polinukleotidinės grandinės viduje, hidrolizę. atitikmenys: angl. endonuclease rus. эндонуклеаза …   Chemijos terminų aiškinamasis žodynas

  • endonuclease — An enzyme that cleaves a phosphodiester bond within a DNA strand, forming two smaller strands. See: exonuclease, restriction endonuclease …   Glossary of Biotechnology

  • endonuclease — noun a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes • Hypernyms: ↑nuclease • Hyponyms: ↑restriction endonuclease, ↑restriction nuclease, ↑restriction enzyme …   Useful english dictionary

  • Endonuclease/Exonuclease/phosphatase family — Pfam box Symbol = Exo endo phos Name = Endonuclease/Exonuclease/phosphatase family width = caption = Pfam= PF03372 InterPro= IPR005135 SMART= Prosite = PDOC00598 SCOP = 1ako TCDB = OPM family= 148 OPM protein= 1zwx PDB=PDB3|1i9yA:560 846… …   Wikipedia

  • endonuclease Serratia marcescens — A nuclease (a nucleate oligonucleotidohydrolase) that forms oligonucleotides ending in 5′ phosphates from RNA and DNA; hydrolyzes both double stranded and single stranded polynucleic acids. SYN: nucleate endonuclease …   Medical dictionary

  • endonuclease — noun Date: 1962 an enzyme that breaks down a nucleotide chain into two or more shorter chains by cleaving the internal covalent bonds linking nucleotides compare exonuclease …   New Collegiate Dictionary

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