footprinting

footprinting
A method for determining the area of DNA covered by protein binding; accomplished by nuclease digestion of the protein-DNA complex followed by analysis of the region of DNA protected by the interaction with protein.

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foot·print·ing -iŋ n identification of the binding sites between a specific DNA and a protein by subjecting a complex of the DNA and protein to chemical digestion and comparing the resulting fragments with those obtained by digestion of the same DNA which does not have some of its cleavage sites protected by binding with the protein

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foot·print·ing (footґprint-ing) a technique for determining the location of binding between a protein and a DNA molecule; the two are bound, enzymes or chemicals are used to hydrolyze unbound, unprotected DNA, and the resulting protected DNA fragments can be identified electrophoretically.

Medical dictionary. 2011.

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Look at other dictionaries:

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  • Footprinting — Le Footprinting est la technique consistant à récolter de l information sur des systèmes informatiques et toutes les entités auxquelles ils sont rattachés. Cela est effectué par le biais de plusieurs techniques : Requêtes DNS ;… …   Wikipédia en Français

  • footprinting assay — footprinting …   Medical dictionary

  • footprinting — A technique used to identify the binding site of, for example, a protein on a nucleic acid sequence. The basic principle is to carry out a very limited hydrolysis of the DNA with or without the protein complexed and then to compare the digestion… …   Dictionary of molecular biology

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  • Phylogenetic footprinting — is a technique used to identify transcription factor binding sites (TFBS) within a non coding region of DNA of interest by comparing it to the orthologous sequence in different species. When this technique is used with a large number of closely… …   Wikipedia

  • DNase footprinting assay — DNaseI footprint of a protein binding to a radiolabelled DNA fragment. Lanes GA and TC are Maxam Gilbert chemical sequencing lanes, see DNA Sequencing. The lane labelled control is for quality control purposes and contains the DNA fragment but… …   Wikipedia

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