A method for determining the area of DNA covered by protein binding; accomplished by nuclease digestion of the protein-DNA complex followed by analysis of the region of DNA protected by the interaction with protein.

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foot·print·ing -iŋ n identification of the binding sites between a specific DNA and a protein by subjecting a complex of the DNA and protein to chemical digestion and comparing the resulting fragments with those obtained by digestion of the same DNA which does not have some of its cleavage sites protected by binding with the protein

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foot·print·ing (footґprint-ing) a technique for determining the location of binding between a protein and a DNA molecule; the two are bound, enzymes or chemicals are used to hydrolyze unbound, unprotected DNA, and the resulting protected DNA fragments can be identified electrophoretically.

Medical dictionary. 2011.

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