Sudan black B fat stain

Sudan black B fat stain
a stain used to demonstrate Legionella and fat vacuoles in bacterial cells. A heat-fixed smear is treated with Sudan black B, cleared with xylol, and counterstained with safranin. Fat vacuoles stain blue-black; bacterial cells stain pink.

Medical dictionary. 2011.

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  • Sudan stain — Sudan staining is the use of Sudan dyes to stain sudanophilic substances, usually lipids. Sudan lysochromes (Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B) are used.Sudan dyes have high affinity to fats, therefore they are used to… …   Wikipedia

  • Sudan III — Chembox new Name = Sudan III ImageFile = Sudan III.png ImageSize = 200px ImageName = Sudan III IUPACName = 1 ((4 (phenyldiazenyl)phenyl)diazenyl)naphthalen 2 ol Section1 = Chembox Identifiers CASNo = 85 83 9 SMILES =… …   Wikipedia

  • stain — 1. To discolor. 2. To color; to dye. 3. A discoloration. 4. A dye used in histologic and bacteriologic technique. 5. A procedure in which a dye or combination of dyes and reagents is used to color the constituents of cells and tissues. For… …   Medical dictionary

  • solvent black 3 — Sudan black B a black, fat soluble diazo dye, used as a stain for fats …   Medical dictionary

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