- affinity purification
- the removal of contaminating elements by means of affinity chromatography.
Medical dictionary. 2011.
affinity purification
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Tandem Affinity Purification — The Tandem Affinity Purification (TAP) is a technique for studying protein protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The protein of interest with the TAP tag first binds to beads… … Wikipedia
Affinity chromatography — is a method of separating biochemical mixtures and based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Contents 1 Uses 2 Principle 3 Batch and column setup … Wikipedia
affinity chromatography — n chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column that has been treated with a substance having a ligand for which the macromolecule has an affinity that causes it to be… … Medical dictionary
affinity tag — An amino acid sequence that has been engineered into a protein to make its purification easier. The tag could be another protein or a short amino acid sequence, allowing purification by affinity chromatography. Synonym: purification tag … Glossary of Biotechnology
purification tag — See: affinity tag … Glossary of Biotechnology
Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… … Wikipedia
Multicolumn countercurrent solvent gradient purification — (MCSGP) is a form of chromatography that is used to separate or purify biomolecules from complex mixtures. It was developed at the Swiss Federal Institute of Technology Zürich by Aumann and Morbidelli.[1] The process consists of three to six… … Wikipedia
separation and purification — ▪ chemistry Introduction in chemistry, separation of a substance into its components and the removal of impurities. There are a large number of important applications in fields such as medicine and manufacturing. General principles… … Universalium
Polyhistidine-tag — A polyhistidine tag is an amino acid motif in proteins that consists of at least six histidine ( His ) residues, often at the N or C terminus of the protein. It is also known as hexa histidine tag, 6xHis tag, and by the trademarked name His tag… … Wikipedia
Monoclonal antibodies — A general representation of the methods used to produce monoclonal antibodies. Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same because they are made by identical immune cells that are all clones of a unique… … Wikipedia
