Folin and Wu method

Folin and Wu method
1. (for creatinine) the color produced by the unknown (protein-free blood filtrate or urine) in an alkaline solution of picric acid is compared in a colorimeter with the color produced by a known solution of creatinine or with a standard solution of potassium bichromate. 2. (for creatine plus creatinine) the creatine of a protein-free blood filtrate is changed to creatinine by heating with dilute hydrochloric acid in an autoclave, and the creatinine thus produced together with the preformed creatinine is determined colorimetrically after adding an alkaline picrate solution. 3. (nonprotein nitrogen) the total nonprotein nitrogen in the protein-free blood filtrate is determined by setting free the nitrogen as ammonia by the Kjeldahl process, nesslerizing this ammonia, and comparing with a standard. 4. (for protein-free blood filtrate) lake the blood with distilled water, add sodium tungstate and sulfuric acid, and filter. 5. (for urea) change the urea to ammonia by means of urease, and nesslerize. 6. (for uric acid) uric acid is precipitated from the protein-free blood filtrate or from urine by silver lactate, treated with phosphotungstic acid, and the blue color compared with the color produced by known amounts of uric acid.

Medical dictionary. 2011.

Игры ⚽ Нужно решить контрольную?

Look at other dictionaries:

  • Folin method — 1. (for acetone) aerate the acetone into a solution of sodium bisulfite and then determine the amount of nephelometric comparison with a standard acetone solution using Scott and Wilson reagent. 2. (for amino acids in blood) make 10 mL of protein …   Medical dictionary

  • Otto Folin — in biochemistry lab at McLean Hospital, 1905; photo by A.H. Folsom (Harvard) …   Wikipedia

  • Gallic acid — IUPAC name …   Wikipedia

  • List of organic reactions — Well known reactions and reagents in organic chemistry include Contents: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z    See also   Ext …   Wikipedia

  • Ácido gálico — El ácido gálico es un ácido orgánico también conocido como ácido 3,4,5 trihidroxibenzoico, que se encuentra en las agallas, en las hojas de té, en la corteza de roble y otras plantas. La fórmula química es C6H2(OH)3COOH. El ácido gálico se… …   Wikipedia Español

  • Molybdenum blue — is a term applied to: reduced heteropolymolybdate complexes, polyoxometalates containing Mo(V), Mo(VI), and a hetero atom such as phosphorus or silicon reduced isopolymolybdate complexes, polyoxometalates containing Mo(V), Mo(VI) formed when… …   Wikipedia

  • reaction — 1. The response of a muscle or other living tissue or organism to a stimulus. 2. The color change effected in litmus and certain other organic pigments by contact with substances such as acids or alkalies; also the property that such substances… …   Medical dictionary

  • test — 1. To prove; to try a substance; to determine the chemical nature of a substance by means of reagents. 2. A method of examination, as to determine the presence or absence of a definite disease or of some substance in any of the fluids, tissues,… …   Medical dictionary

  • Assay — An assay is an analysis done to determine: {{}}The presence of a substance and the amount of that substance. Thus, an assay may be done for example to determine the level of thyroid hormones in the blood of a person suspected of being hypothyroid …   Medical dictionary

  • Polyphenol antioxidant — A polyphenol antioxidant is a type of antioxidant containing a polyphenolic substructure. In human health these compounds, numbering over 4000 distinct species, are thought to be instrumental in combating oxidative stress, a syndrome causative of …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”